The word Enzyme brings to mind the enzymes studied in biology like trypsin and Insulin. However, as is common knowledge, the uses of enzymes are not only restricted to biological systems but they are also used widely in the industry. Biotechnology, with its knowledge intensive nature and tremendous economic potential, has emerged as one of the rapidly growing sectors of the Indian knowledge economy today. Focusing on the practical use of biological systems to produce goods and services, biotechnology has made significant achievements in the growth and its application in the areas of agriculture, healthcare, environment, etc through R & D projects and infrastructure creation. Uses of Enzyme The food processing industry is highly dependent on enzymes. During processing of food, enzymes are added to elaborate a wide range of effects. One such important effect is to modify biopolymers to obtain the desired end product. Further, the action of enzymes on foodstuffs facilitates in improving digestibility, palatability and attractiveness besides enhancing nutritive value of the food. The main user industries are detergents, starch, alcohol, protein, fruit Juice, food storage, in dairy processing, in antibiotics production etc, each with around 30% of sales, and dairy with about 15%. The remaining sales are distributed in an extremely diverse range of industries. Market Survey The industries involved in enzyme production in India is almost nil when compared to the world enzyme market, though in certain cases, considerable amounts of raw materials are supplied for their enzyme production. In the industries, either submerged culture or surface culture techniques are used for enzyme production. While the Japanese enzyme producers employ the relatively simple but labour intensive techniques of surface culture, USA and European producers use the submerged culture approach. Instead of importing the technical know how from Western countries, it is desirable to develop the same suitable to our needs, keeping in view the existing infrastructural facilities available here. Enzyme industry will flourish only when it realizes the fact that the present development of various other chemical industries are at the expense of the future generation. Unless the society and in turn policy makers realize the importance of the use of enzymes, which is an eco friendly process, it is difficult to predict the potential for enzyme industry in our country. Based on growth rate in India, the demand for enzyme is computed which is definitely higher than the production. Enzyme technology is presently going through metamorphosis. It is the development of the theory concerning enzymic function and how the structural entity of various domains of a protein are revealed to its catalytic activities. Still there remains, for further development of a useful process based on the present understanding of enzyme. In future, enzymes will be used more widely for diverse actions. Established enzymes will be put to new uses in different media and novel enzymic functions would emerge out of this process. Few Indian Major Players are as under: Advanced Enzyme Technologies Ltd. Anil Bioplus Ltd. Concord Biotech Ltd. Fermenta Biotech Ltd. Precise Laboratories Pvt. Ltd.