Fructose is a simple monosaccharide found in many foods. It is a white solid that dissolves readily in water. Honey, tree fruits, berries, melons and some root vegetables, contain significant amounts of the fructose derivative sucrose (table sugar). Sucrose is a disaccharide derived from the condensation of glucose and fructose. Crystalline fructose and high-fructose corn syrup are often confused as the same product. Crystalline fructose, which is often produced from a fructose-enriched corn syrup, is indeed the monosaccharide. High-fructose corn syrup, however, is usually considered to be a mixture of nearly equal amounts of fructose and glucose. High fructose corn syrup provides sweetness intensity equivalent to sugar. High fructose corn syrup can replace sugar in one-for-one proportions. The sweetness profile of high fructose corn syrup enhances many fruit, citrus and spice flavors in beverages, bakery fillings and dairy products. High fructose corn syrup is composed of either 42 percent or 55 percent fructose, with the remaining sugars being primarily glucose and higher sugars. In terms of composition, high fructose corn syrup is nearly identical to table sugar (sucrose), which is composed of 50 percent fructose and 50 percent glucose. Glucose is one of the simplest forms of sugar that serves as a building block for most carbohydrates. Fructose is a simple sugar commonly found in fruits and honey. High fructose corn syrup is used in foods and beverages because of the many benefits it offers. In addition to providing sweetness at a level equivalent to sugar, High fructose corn syrup enhances fruit and spice flavors in foods such as yogurt and spaghetti sauces, gives chewy breakfast bars their soft texture and also protects freshness. High fructose corn syrup keeps products fresh by maintaining consistent moisture. The industry responses towards substitution of sugar by HFS are positive provided a) it does not interfere with the product quality; b) regular supply of HFS is ensured; c) its use offers a price advantage over sugar; d) it does not change colour on heating; e) preservative qualities for the products is as good as in the case of sugar; f) there is no objection in using HFS by FPO or other food related enforcement agencies. HFS can replace sugar in industrial and domestic applications. Among industries, the major consuming sectors include bakery, confectionery, processed foods, beverages, soft drinks, ice creams, baby foods. Now-a-days the demand for HFCS is increasing day by day, so there is wide scope for new entrepreneurs to venture into this project.